A New Paradigm for Ovarian Sex Cord-Stromal Tumor Development

Abstract

Transforming growth factor beta (TGFB) family members regulate multiple cellular functions and key reproductive processes in a contextually dependent manner via the interaction with membrane associated serine/threonine kinase receptor complexes (TGFBR1/TGFBR2) and downstream SMAD proteins. To complement our mouse model containing a constitutively active TGFBR1 using growth differentiation factor 9 (Gdf9)-Cre (i.e., TGFBR1-gCA), we herein generated a mouse model using Zp3-Cre line (termed TGFBR1-zCA). We performed a number of experiments including H and E staining, immunohistochemistry, and apoptosis assay to analyze potential ovarian phenotype of these mice. In contrast to TGFBR1-gCA mice, the TGFBR1-zCA mice did not develop ovarian tumors and demonstrated essentially normal ovarian histology and expression of granulosa cell and germ cell proteins. Using ovarian RNA from TGFBR1-gCA mice and controls, we performed RNA-seq experiment. Initial analysis identified 1301 genes that were differentially regulated in the TGFBR1-zCA ovaries versus controls. Interestingly, a number of genes are associated with folliculogenesis and oogenesis. Further studies will be focused on exploiting the RNAseq data and defining key regulators and/or pathways for ovarian tumor development.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2017
Accession Number
AD1046813

Entities

People

  • Qinglei Li

Organizations

  • Texas A&M University

Tags

DTIC Thesaurus Topics

  • Apoptosis
  • Biological Staining And Labeling
  • Cells
  • Data Analysis
  • Data Mining
  • Genes
  • Genetics
  • Germ Cells
  • Growth Factors
  • Histology
  • Immunohistochemistry
  • Neutral Amino Acids
  • Peptide Growth Factors
  • Peptides
  • Phenotypes
  • Proteins
  • Regulators

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.
  • Molecular Biology and Genetics
  • Oncology (Cancer Research).