Targeting Tryptophan Catabolism: A Novel Method to Block Triple-Negative Breast Cancer Metastasis

Abstract

Triple negative breast cancer (TNBC) cells upregulate the kynurenine pathway (KP) in forced suspension culture. The rate limiting enzyme in this pathway, TDO2 is responsible for tryptophan catabolism and production of the metabolite kynurenine (Kyn). Kyn was recently identified as an endogenous ligand for AhR, a transcription factor that was also upregulated in suspension. Kyn activation of AhR promotes motility of glioma cells. AhR is also in many immune cell types and its activation decreases T-cell activity leading to tumor immune escape. The goal of our proposal is to determine if we can target this pathway that may facilitate TNBC metastasis by enabling tumor cell invasiveness, anchorage independence and immune escape. Our hypothesis is that upregulation of kynerinine by TNBC facilitates survival in transit to metastatic sites and immune suppression and thereby mediates the highly metastatic nature of this subtype.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2017
Accession Number
AD1046835

Entities

People

  • Jennifer K Richer

Organizations

  • University of Colorado Boulder

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Blood
  • Breast Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Media
  • Culture Techniques
  • Gene Expression
  • Liquid Chromatography
  • Lymphatic System
  • Lymphocytes
  • Medical Personnel
  • Metabolism
  • Molecules
  • Neoplasms
  • Proteins

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular and Cellular Biology