Small Molecule Protection of Bone Marrow Hematopoietic Stem Cells

Abstract

During the above project period we have demonstrated the following progress on Aims 1 and 2, and have keyed the following results to specific major tasks under each Aim: 1. mitotic expansion of human mobilized peripheral blood CD34 positive hematopoietic stem cells (HSCs) in culture in defined medium supplemented with hematopoietic growth factors and small molecule inhibitors of differentiation; 2. suppression of differentiation of CD34 positive CD38 negative CD90 positive during mitotic expansion in culture by addition of the small molecule inhibitors SR-1 or UM-171, alone or in concert; 3. dose-dependent killing of CD34 positive CD38 negative CD90 positive HSCs by the addition of formaldehyde; 4. development of sensitive and specific mass spec assays for DNA adducts and DNA-protein crosslinks (DPCs) generated by formaldehyde in human cells.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2015
Accession Number
AD1047146

Entities

People

  • Raymond J. Jr Monnat

Organizations

  • University of Washington

Tags

DTIC Thesaurus Topics

  • Aldehydes
  • Biomedical Research
  • Bone Marrow
  • Cell Line
  • Cells
  • Consortiums
  • Department Of Defense
  • Formaldehyde
  • Gene Expression
  • Liquid Chromatography
  • Maryland
  • Mass Spectrometry
  • Molecules
  • Professional Development
  • Proteins
  • Small Molecules
  • Stem Cells

Fields of Study

  • Chemistry
  • Medicine

Readers

  • Immunology and Pathology
  • Molecular Biology and Genetics
  • Solar Photovoltaics and Thermoelectric Devices.

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech