Proteomic Analysis of Trauma-Induced Heterotopic Ossification Formation

Abstract

This four-year Mentored IDEA proposal examined the proteome of serum samples derived from civilian and military orthopedic trauma patients and evaluated this information based on the occurrence of heterotopic ossification (HO). Parallel studies of primary human stromal/stem cells were examined in vitro to evaluate the underlying biochemical mechanism of HO formation. These outcomes implicated a number of pathways relating to the mTOR, collagen adhesion molecules, plasminogen, and the fos/fra transcription factors as contributory to HO formation. These studies were extended to a rat blast injury model of HO which added confirmatory evidence in support of the human serum and stromal/stem cell based findings. This information provides avenues for further exploration of HO pathophysiology.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 2017
Accession Number
AD1047608

Entities

People

  • Jeffrey M. Gimble

Organizations

  • Tulane University of Louisiana

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Blast Injuries
  • Cell Physiological Processes
  • Cells
  • Computer Programs
  • Diseases And Disorders
  • Health Services
  • Heterotopic Ossification
  • Mass Spectroscopy
  • Military Medicine
  • Molecules
  • Osteogenesis
  • Physiology
  • Proteins
  • Stem Cells
  • Tissues
  • Transcription Factors

Readers

  • Molecular Biology and Genetics
  • Regression Analysis.
  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Biotechnology