The Role of an Aggrecan 32mer Fragment in Post-Traumatic Osteoarthritis

Abstract

During this reporting period we completed an 8 week DMM experiment, in the presence and absence of the AF-28 antibody. This experiment revealed a trend for an AF-28-mediated reduction in knee hyperalgesia, but not mechanical allodynia, in mice at 8 weeks post-surgery. This trend was not statistically significant, however, we are looking forward to results from the next time point, at 16 weeks post-surgery. The hind limbs from the 8 week study, from mice with and without AF-28 treatment, have been shipped to Australia and will now be analyzed for changes in cartilage, bone and synovial structure. In addition, we have made good progress on developing an AlphLISA immunoassay for detecting 32mer in sera, and we are not far away from final ethics approval to test sera in this new assay.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2017
Accession Number
AD1047656

Entities

People

  • Amanda Fosang
  • Anne-marie Malfait

Organizations

  • University of Melbourne

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Arthritis
  • Australia
  • Cartilage
  • Cells
  • Connective Tissue Cells
  • Diseases And Disorders
  • Dynamic Range
  • Health Services
  • Immunoassay
  • Institutional Review Board
  • Medical Personnel
  • Molecules
  • Osteoblasts
  • Peptides
  • Professional Development
  • Surgery

Fields of Study

  • Medicine

Readers

  • Immunology
  • Neurotrauma and Rehabilitation Medicine.
  • Regression Analysis.