Mouse and Human Models for Investigating Influences of Tau on Progression of Alzheimer's Disease Following Traumatic Neuronal Injury
Abstract
We have completed the design, fabrication, and validation of a new biomedical device to impose moderate mechanical loads on cultured stem-cell derived neuronal and glial cells. Using this device, we assessed morphological changes, beta amyloid production, and tauphosphorylation (i.e., multiple Alzheimer's-associated outcomes) following rapid stretch of iPSC-derived neurons. Results suggest that neurites oriented in the direction of substrate stretch were non-elastically deformed, the cytoskeleton was reorganized, a myloid precursor protein transport was perturbed, amyloid production was increased, and tau phosphorylation unchanged following a single bout of mechanical loading. Multiple bouts of mechanical loading amplified amyloid and tau phenotypes, suggesting a dependence of these Alzheimer's associated outcomes to injury dose or severity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2017
- Accession Number
- AD1048149
Entities
People
- Sameer B Shah
Organizations
- University of California, San Diego