Does the Loss of Stromal Caveolin-1 Remodel the Tumor Microenvironment by Activating Src-Mediated PEAK1 and PI3K Pathways

Abstract

This study describes a new mechanism of intercellular communication originating from extracellular vesicles (EVs). We demonstrate that in the context of prostate cancer, EV populations isolated from human patients harbor AKT1 and that AKT1 kinase activity is sustained in these particles, nominating them as active signaling platforms. Consistently, active AKT1 in circulating EVs from the plasma of metastatic prostate cancer patients is detected predominantly in large, tumor-derived EVs, termed large oncosomes (LO). LO internalization induces reprogramming of human normal prostate fibroblasts, as reflected by high levels of -SMA, IL6, and MMP9. In turn, LO reprogrammed normal prostate fibroblasts stimulate endothelial tube formation in vitro.

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Document Details

Document Type
Technical Report
Publication Date
Nov 01, 2017
Accession Number
AD1048478

Entities

People

  • Dolores Di Vizio
  • Mariana R. Sobreiro
  • Michael R Freeman
  • Wei Yang

Organizations

  • Cedars-Sinai Medical Center

Tags

DTIC Thesaurus Topics

  • Antineoplastic Agents
  • Biological Factors
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Confocal Microscopy
  • Culture Media
  • Health Services
  • Lymphocytes
  • Medical Personnel
  • Peptide Growth Factors
  • Proteins
  • Stem Cells

Fields of Study

  • Biology

Readers

  • Electronics Engineering
  • Molecular Biology and Genetics
  • Prostate Cancer Biology.