Development and Validation of a Simplified Renal Replacement Therapy Suitable for Prolonged Field Care in a Porcine (Sus scrofa) Model of Acute Kidney Injury
Abstract
Objectives: To validate a RRT system compatible with prolonged field care (PFC). We hypothesized that our improvised RRT (IRRT) system would have similar performance to conventional RRT (CRRT). Methods: Twelve swine were anesthetized. A dialysis catheter was introduced. Bilateral nephrectomies were performed. The aorta was occluded in Zone 3 for 2 hours. Animals were randomized to CRRT or IRRT. In the IRRT group, the arterial line of the dialysis catheter was connected to a Belmont rapid infuser which was then connected to a Gambro dialysis cartridge, and subsequently to the venous line of the dialysis catheter. Replacement fluids were made with stock solutions. At the end of the 2-hour occlusion, animals received 4 hours of RRT. Animals were treated with isotonic crystalloids and norepinephrine to maintain their mean arterial pressure. Results: There was no difference in serum creatinine, electrolytes, dialysate or ultra filtrate volumes between the groups. There was no difference between the commercial and the custom-made replacement fluids. There was no difference in serum lactate, isotonic crystalloids, or norepinephrine required for resuscitation. Conclusion: The IRRT system achieved similar performance as the CRRT. The IRRT system may prove helpful for the treatment of severe AKI in PFC.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2018
- Accession Number
- AD1048701
Entities
People
- Guillaume L. Hoareau
Organizations
- David Grant USAF Medical Center