Biological Characterization and Clinical Utilization of Metastatic Prostate Cancer-Associated lincRNA SchLAP1
Abstract
During the research period, we followed our proposal to characterize the biological function of SChLAP1 in prostate cancer and assess the translational potential of SChLAP1 in prostate cancer. To ascertain the potential protein binding sites of SChLAP1, we performed PIP-seq in prostate cancer cells, and found SChLAP1 Exon 5 is the most protected exonic region. Then, we performed smFISH assay to precisely characterize the subcellular localization pattern of SChLAP1 at single molecule level. We found that SChLAP1 is highly nuclear specific transcript, and the absolute counts of SChLAP1 molecules was ascertained in prostate cancer cell line as well as prostate cancer biopsies. To functional annotate the interaction between SChLAP1 and SWI/SNF complex, we performed MNase-seq to determine the effect of SChLAP1 on global nucleosomes occupancy changes, we found that overexpression of SChLAP1 in prostate cells significantly affectsnucleosome position around the genes with FPKM >1. Gene ontology (GO) analysis suggest that cell-cell adherens and cell cycle regulation are the most affected gene sets by SChLAP1. To sum up, our recently findings have substantial impact on understanding the biological function of SChLAP1 in prostate cancer progression, and helped us to further explore the potential of SChLAP1 to serve as biomarkers and therapeutic target for metastatic prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2017
- Accession Number
- AD1050190
Entities
People
- Lanbo Xiao
Organizations
- Board of Regents of the University of Michigan