Discovery and Characterization of PRCAT47: A Novel Prostate Lineage and Cancer-Specific Long Noncoding RNA

Abstract

The overall goal of this project is to characterize the functioning mechanism, utility and therapeutic potential of the biomarker PRCAT47 in prostate cancer. During this first period, we investigated behind regulation of androgen receptor by PRCAT47, we developed tissue microarrays (TMAs) representing patients with clinically localized prostate cancer and metastatic castrate resistant prostate cancer, and we optimized a novel RNA in situ hybridization technology to allow us to assess PRCAT47 expression in prostate tissues. We also assessed the therapeutic potential of clinical grade anti-sense oligonucleotides (ASOs) targeting PRCAT47. ASOs were able to knock down PRCAT47 at high efficacy. Genes regulated upon ASO-mediated knockdown are highly correlated with that of siRNA-mediated knockdown. Moreover, treatment of ASOs delays prostate cancer cell growth in vitro, laying a foundation for future experiments in vivo.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2017
Accession Number
AD1050260

Entities

People

  • Rohit Mehra

Organizations

  • Board of Regents of the University of Michigan

Tags

DTIC Thesaurus Topics

  • Androgen Receptors
  • Androgens
  • Biological Markers
  • Biomolecules
  • Cell Line
  • Chemistry
  • Diseases And Disorders
  • Gene Expression
  • Hybridization
  • Molecules
  • Neoplasms
  • Prostate Cancer
  • Proteins
  • Regulations
  • Rna Stability
  • Targeting
  • Tissues

Fields of Study

  • Biology

Readers

  • Oncology (Cancer Research).
  • Oncology and Biomarker-Based Cancer Detection.
  • Prostate Cancer Biology.