Systems Biology of the Immune Response to Live and Inactivated Dengue Virus Vaccines
Abstract
The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive (T and B cell) responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the first project year, IRB approvals, inter-institutional agreements, and measurements of dengue virus-specific neutralizing antibody titers and frequencies of cytokine-producing CD4 and CD8 T cells were completed. Flow cytometry of cellular activation and measurement of serum cytokine levels were completed on a subset of subjects. Pilot studies were done for RNA extraction and analysis of B cells and T cell repertoires.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2017
- Accession Number
- AD1050422
Entities
People
- Alan L. Rothman
- Jeffrey R. Currier
Organizations
- University of Rhode Island