In-Depth Analysis of Citrulline-Specific CD4 T-Cells in Rheumatoid Arthritis
Abstract
The goal of this project is to test the hypothesis that cit-specific CD4 T cells present in rheumatoid arthritis (RA) patients exhibit a distinct cell surface phenotype andtranscriptional signature that could be used to predict disease, response to therapy and identify novel therapeutic targets for the treatment of RA. In Year 3, we havecontinued to make significant progress for all our goals. For Aim 1, our findings suggest that the dominant autoantigen driving disease may differ between individuals and that the character of the inflammatory response in RA may be linked to the antigen during the CD4 T cell response. Furthermore, the dominant autoantigen driving disease ultimately may determine the response to therapy. For Aim 2, our analysis of the whole blood RNA sequencing dataset suggest that T cell exhaustion is dysregulated in subjects carrying the HLA-DRB1 alleles associated with increased risk of RA. A twelve-month no-cost extension was recently approved to allow us to do additional bioinformatics analysis to strengthen these results for publication. The no-cost extension was also approved for additional time to complete both the RNA sequencing of antigen-specific T cells in Aim 2 and the ex vivo tetramer analysis of the samples from the longitudinal cohort in Aim 3.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2018
- Accession Number
- AD1050556
Entities
People
- Bernard Ng
- Jane H Buckner
Organizations
- Seattle Institute for Biomedical and Clinical Research