Development and Validation of a Porcine (Sus scrofa) Sepsis Model
Abstract
Objectives: We sought to develop and validate a porcine model of a sepsis-like state suitable for the future study of EPACC in sepsis. Specifically, we aimed at determining the proper LPS loading dose and constant rate infusion regimen to achieve a sepsis-like state. Methods: Animals were anesthetized and instrumented for cardiovascular monitoring. Lipopolysaccharide (LPS, a large molecule present on the outer layer of Gram negative bacteria) (Sigma Aldrich, ready-made solution, L5293) was injected at various dosing regimen (with or without loading dose). Animals cardiovascular parameters (heart rate, mean arterial blood pressure) were monitored continuously. Results: We observed that animals had unpredictable responses to LPS injection and could not identify a protocol that would be reproducible. For a given dose we would observe some pigs displaying little to no response while others may suffer decompensated vasodilatory shock leading to death.Conclusion: LPS injection is not a suitable model for our research effort. We are currently validating a live bacteria infusion protocol to induce sepsis in pigs.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2018
- Accession Number
- AD1050559
Entities
People
- Guillaume L. Hoareau
Organizations
- David Grant USAF Medical Center