Probing Enzyme-Surface Interactions via Protein Engineering and Single-Molecule Techniques

Abstract

The overall objective of this research is to exploit protein engineering and fluorescence single molecule methods to enhance our understanding of the interaction of proteins and surfaces. Given this objective, the specific aims of this research are to: 1) exploit the incorporation of unnatural amino acids in proteins to introduce single-molecule probes (i.e., fluorophores for fluorescence resonance energy transfer (FRET) measurements), 2) demonstrate the utility of site-specific labeling for characterizing surface-induced conformational-bias of proteins at the single-molecule level, and 3) correlate changes in protein structure and interfacial dynamics (i.e., adsorption, diffusion, and desorption) upon interaction of freely diffusing proteins with surfaces with polar, non-polar, and polymer chemistries.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Jun 26, 2017
Accession Number
AD1050666

Entities

People

  • Joel L Kaar

Organizations

  • University of Colorado Boulder

Tags

Communities of Interest

  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Amino Acids
  • Chemical Synthesis
  • Chemistry
  • Dynamics
  • Energy Transfer
  • Engineering
  • Fluorescence
  • Fluorophores
  • Hydrophobic Properties
  • Materials
  • Materials Science
  • Molecular Dynamics
  • Molecules
  • Physical Properties
  • Polymer Chemistry
  • Polymers
  • Protein Engineering

Fields of Study

  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Nanoscale Plasmonic Nanotechnology

Technology Areas

  • Biotechnology