NAU-TGen North Dangerous Pathogens DNA Forensics Center Upgrade

Abstract

Despite its importance for infectious disease diagnosis, the ability to rapidly identify the causative agent for infections remains an elusive. Whole genome sequencing for clinical diagnostics is emerging as the future of disease identification and management by providing a rapid and highly sensitive method of diagnosing and characterizing infection. We have created a metagenomic application for analysis of clinical samples titled MetaGenomics Explorer (MetaGeniE, previously called IRIMAS)that is undergoing optimization and statistical validation with a range of different datasets. MetaGeniE provides novel insights and analyses of clinical datasets, detecting and characterizing the major component of community that is usually the infectious agent of interest as well as identifying the other microbes in this community. Furthermore, we are able to validate these detections with laboratory culture, and available patient information. Finally, we are able to take these analyses a step beyond nearly all current approaches and can use both SNP genotyping and determine features such as antibiotic resistance to precisely characterize these pathogens.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 2014
Accession Number
AD1052106

Entities

People

  • Arun Rawat
  • Betty Diamond
  • James Schupp
  • Jeffrey T. Foster
  • Paul Keim
  • Rebecca E Colman

Organizations

  • Northern Arizona University

Tags

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Bacteria
  • Bacterial Infections
  • Bacteriology
  • Chromosomes
  • Coinfection
  • Disease Outbreaks
  • Escherichia Coli
  • Genetics
  • Infection
  • Infectious Diseases
  • Microbiology
  • Microbiomes
  • Microorganisms
  • Mycobacterium Tuberculosis
  • Pathogenic Bacteria

Fields of Study

  • Biology

Readers

  • Infectious Disease/Epidemiology
  • Molecular and genetic basis of cancer.
  • Oncology and Biomarker-Based Cancer Detection.