Optimized Liposomal Dexamethasone Therapy Improves Functional Outcome of Post-Traumatic Skeletal Muscle and Neuromuscular Junction
Abstract
In this fiscal year (03/01/2017 to 02/28/2018), we performed our research experiments in four groups of mice including sham, tourniquet-induced IR+saline, tourniquet-induced IR+lipo-Dex, and tourniquet-induced IR+Dex groups. These experiments tested the protective effects of lipo-Dex and Dex on skeletal muscle morphology and function in mice with tourniquet-induced IR. Lipo-Dex is liposome-encapsulated Dex. Our study found that lipo-Dex was mainly retained in IR-injured skeletal muscles after 24-hour ischemia/reperfusion (IR), lasted about 1 week, and then disappeared. Unilateral (left) hindlimb ischemia was induced in IR mice by placing an orthodontic rubber band (ORB) at the hip joint through use of a McGivney hemorrhoidal ligator. After 3 hours of ischemia, the ORB tourniquet was released to begin reperfusion for different periods. Treatment with Dex (1mg/kg/day, i.p. injection for 1 wk) or lipo-Dex (7mg/kg, one-time i.v., equivalent to 1 mg/kg/day of Dex for 1 wk) will begin at the beginning of reperfusion on the day of IR induction. Our data demonstrated that treatment of lipo-Dex or Dex suppresses IR-induced inflammation and promotes recovery of skeletal muscle morphology and function from tourniquet-induced IR injury.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2018
- Accession Number
- AD1052122
Entities
People
- Yulong Li
Organizations
- University of Nebraska Medical Center