Understanding the Interaction of Peptides and Proteins with Abiotic Surfaces: Towards Water Free Biologics
Abstract
The major goals presented in the original proposal: Aim 1. Develop systematic understanding of abiotic/biotic interfaces. We aim to understand, how the structure and activity of proteins and peptides immobilized on abiotic surfaces depends upon a) the chemical and physical nature of the abiotic surface; b) the hysicochemical properties of the protein or peptide; and c) the nature of the surrounding medium water, air or other hydromimetic molecules. Aim 2. Develop design rules for water-free biologics based on engineered abiotic/biotic interfaces. Using knowledge gained from studies in Aim 1, we aim to a) engineer peptides and proteins with enhanced ability to retain structure and function in the absence of bulk water, and b) develop abiotic surfaces displaying hydromimetic functionality that stabilizes the native structure and function of biological molecules in extremely low humidity or water-free interfaces i.e. a surface-air interface that lacks bulk water and in air that is not humidified.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 18, 2018
- Accession Number
- AD1053734
Entities
People
- Charles L Brooks
- Neil Marsh
- Nick Abbot
- Zhan Chen
Organizations
- University of Michigan