Probe Beam Deflection Optical Imaging of Thermal and mechanical Phenomena Resulting from Nanosecond Electric Pulse (nsEP) Exposure In-Vitro
Abstract
The biological effects of nanosecond-duration electric pulses (nsEP) have been studied extensively and are theorized to induce temporary nanoporation of plasma membranes of eukaryotic cells in vitro and in vivo. The physical to biological driving mechanisms behind nanoporation still remain unclear, but could be a result of a multitude of stimuli, including mechanical (shockwaves, electrode formation), thermal, and electrical (ion transport, channel gating). Experimentally quantifying and characterizing mechanical fields with piezoelectric transducers proves difficult due to electromagnetic interference (EMI) generated by nsEP pulses. The probe beam deflection technique is a passive optical method for quantifying refractive index gradients in liquids and gases that probes both dynamic and static events. In this work, a probe beam deflection imaging system was designed to acquire 2-D images of thermal/mechanical near and far field phenomena resulting from monopolar and bipolar nsEP pulses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 14, 2017
- Accession Number
- AD1053866
Entities
People
- Bennett L. Ibey
- Caleb C Roth
- Christopher Valdez
- Erick Moen
- Gary Noojin
- Hope T. Beier
- Joel N. Bixler
- Mehdi Shadaram
- Ronald A. Barnes