Prevention of Breast Cancer Skeletal Metastases with Parathyroid Hormone

Abstract

During this research period we have demonstrated that parathyroid hormone (PTH) started at the same time as intramammary implantation of murine 4T1-BGL breast cancer cells can decrease metastases to the skeleton but not to lung, liver or spleen. We have further demonstrated that 4 weeks of pretreatment with PTH can decrease engraftment and growth of murine or human breast cancer cells injected into the in tratibial bone marrow cavity. PTH also decreases the migration of breast cancer cells towards osteoblasts in a Transwell assay. Some of the effects of PTH are mediated by decreasing expression of VCAM-1 in osteoblasts. Vcam1 mRNA andVCAM-1 protein are decreased in bones treated with PTH. Breast cancer cells engineered to overexpress VCAM-1 increase migration towards osteoblasts, and this is blunted by PTH treatment.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 2018
Accession Number
AD1053915

Entities

People

  • Joy Y. Wu

Organizations

  • Stanford University

Tags

DTIC Thesaurus Topics

  • Biological Sciences
  • Blood
  • Body Weight
  • Bone And Bones
  • Bone Diseases
  • Bone Marrow
  • Breast Cancer
  • Cancer
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Culture Techniques
  • Diseases And Disorders
  • Experimental Design
  • Gene Expression
  • Lymphatic Diseases
  • Medical Personnel
  • Neoplasms
  • Oncology
  • Osteogenesis
  • Parathyroid Hormones
  • Peptide Growth Factors
  • Proteins
  • Stem Cells
  • Therapy
  • Tissues

Fields of Study

  • Chemistry

Readers

  • Immunology and Pathology
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology