The Effects of Ionizing Radiation and Oxidizing Species on Strains of Deinococcus Radiodurans Lacking Endogenous Oxidative Protection Methods

Abstract

Multiple strains of Deinococcus radiodurans were transformed, creating knockout mutations in genes responsible for manganese ion transport, manganese and copper/zinc super-oxide dismutase, and bacillithiol synthesis. These mutated strains were then irradiated with ~20,000 Gys. The results showed that the mutated strains had a higher sensitivity to ionizing radiation, some having an increase in sensitivity 3000 times more than wild type Deinococcus radiodurans. In addition to radiation the mutated strains were also exposed to paraquat, an oxidizing herbicide. Strains missing manganese super-oxide dismutase and bacillithiol synthesis showed increased sensitivity.

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Document Details

Document Type
Technical Report
Publication Date
Mar 24, 2016
Accession Number
AD1054219

Entities

People

  • Dylan L. Klawuhn

Organizations

  • Air Force Institute of Technology

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Air Force
  • Cell Membrane
  • Cell Physiological Processes
  • Cells
  • Chemical Reactions
  • Deoxyribonucleic Acids
  • Eukaryotes
  • Free Radicals
  • Gamma Rays
  • Gel Electrophoresis
  • Health Services
  • Ionization
  • Ionizing Radiation
  • Linear Accelerators
  • Oxidative Stress
  • Polymerase Chain Reaction
  • Radiation

Readers

  • Materials Science (Mechanical Engineering).
  • Molecular Genetics
  • Nuclear and Radiation Engineering.