Scaling Up Complexity of DNA Brick Structures
Abstract
DNA-based assemblies of increasing complexity may allow for novel applications in vitro and in vivo. Here, we increase the number of unique, addressable components in DNA Brick structures by 100-fold, from hundreds to 10,000 - for a molecular weight of 1 GDa. We also create relatively flat, micron-scale structures from these bricks using a repeating crystal approach, and apply the results to patterning carbon nanotubes for the electronics industry to help meet ever-increasing speed and efficiency targets. Further, we also develop single-stranded DNA and RNA Origami approaches, which allow for rapid copying and robust assembly of large structures. Finally, we develop in vivo structures capable of controlling RNA translation with levels of precision not yet seen in synthetic biology, and combine orthogonal signal comrol to execute complex logical functions.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 12, 2017
- Accession Number
- AD1056267
Entities
People
- Peng Yin
Organizations
- Harvard Medical School