Exogenic Human Heart In Gene Edited Animals
Abstract
Congenital Heart Disease and heart failure are deadly. Cardiac transplantation is the only cure for heart failure and demand for hearts is significantly greater than the supply of donor hearts. The long range goal of this proposal is the production of a humanized heart using NKX2-5/HANDII/TBX5 knockout pigs as hosts for production. These animals may ultimately serve as an organ source for heart transplantation into humans. In addition to serving as a novel source of human hearts for the treatment of heart disease, the humanized pigs will also serve as a large animal model to study the regeneration of the human heart or response(s) to pharmacological agents or novel devices. During the second year of DOD funding we have established a productive cloning laboratory. We have examined multiple hiPSC lines regarding their ability to survive, proliferate and differentiate following delivery to the porcine parthenote blastocysts. Finally, we have initially examined the hiPSC-porcine parthenote chimeras following delivery to pseudopregnant gilts. This strategy has the capacity to have a profound impact on the development of emerging therapies for chronic cardiovascular diseases and transplantation that will benefit our military personnel, veterans and their families.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2017
- Accession Number
- AD1056728
Entities
People
- Daniel J Garry
Organizations
- Regents of the University of Minnesota