Development of a Non-Human Primate (Rhesus Macaque) Model of Upper Limb Vascularized Composite Allotransplants
Abstract
Background: Traumatic limb amputations represent a substantial challenge facing many civilian and combat wounded. Despite advancements in VCA research to date, a tissue preservation strategy that successfully mitigates ischemia reperfusion injury, preserves graft functionality, and provides long-term graft survival remains imperfect. Objective: To develop a non-human primate (NHP) model of upper limb vascularized composite allotransplant, that allow for evaluation of tissue preservation and reperfusion strategies. Materials and methods: Upper extremities were procured from male rhesus macaques (n=17). Cold storage preservation with University of Wisconsin solution for 12 hours at 4 degrees Celsius was performed, followed by reperfusion of the limbs using extracorporeal membrane oxygenation and a pulsatile perfusion pump with warm autologous whole blood. Results: The results shown here are gathered from n=5 extremities. Significant changes: (1) compared to BSLN lactate and potassium were significantly increased at R60, (2) Base deficit significantly decreased at R0 and R60, (3) Cytokines IL-8 and sCD40-L were significantly increased at R60. Histopathological analysis improved after reperfusion. Conclusions: This protocol establishes a baseline for a standardized NHP limb preservation and is the first step in developing an ex vivo pre-transplantation assessment tool providing statistical analysis of projected graft function and survival.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 15, 2018
- Accession Number
- AD1057224
Entities
People
- Forest R. Sheppard
- Jacob J. Glaser
- Michael R. Davis
- Nicholas L. Robbins
- Rodriguez J. Gerardo
- Ruth M. Paredes
Organizations
- Naval Medical Research Unit—San Antonio