Final Report: Characterization of Atmospheric Biological Particles Using Confocal Raman Spectroscopy and Optical Trapping
Abstract
We have developed new techniques for characterization of atmospheric biological particles using confocal Raman spectroscopy and optical trapping. Achievements include: (1) developed optical pulling technique for single light-absorbing particles and smut spores in air over a meter-scale distance using a single collimated laser beam based on negative photophoretic force; (2) characterized optical trapping and rotation of airborne absorbing particles with a single focused laser beam; (3) developed ultralow frequency Stokes anWe have developed new techniques for characterization of atmospheric biological particles using confocal Raman spectroscopy and optical trapping. Achievements include: (1) developed optical pulling technique for single light-absorbing particles and smut spores in air over a meter-scale distance using a single collimated laser beam based on negative photophoretic force; (2) characterized optical trapping and rotation of airborne absorbing particles with a single focused laser beam; (3) developed ultralow frequency Stod anti-Stokes Raman spectroscopy for the analysis of single living cells and biomolecules; (4) measured Raman spectra of single airborne absorbing particles trapped by a single laser beam; (5) discovered memory of germinant stimuli in bacterial spores; (6) observed the dynamic germination of single bacterial spores using rapid Raman imaging; (7) characterized uptake of and resistance to the antibiotic berberine by individual dormant, germinating and outgrowing Bacillus spores as monitored by laser tweezers Raman spectroscopy; (8) studied uptake and levels of the antibiotic berberine in individual dormant and germinating Clostridium difficile and Bacillus cereus spores as measured by laser tweezers Raman spectroscopy; and (9) characterized cold atmospheric plasma inactivation of individual bacterial spores using Raman spectroscopy and phase contrast microscopy. These results help the characterization of atmospheric biological particles.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 25, 2017
- Accession Number
- AD1057411
Entities
People
- Yong-qing Li
Organizations
- East Carolina University