Genome Sequencing to Enable a Model Salamander for Tissue Regeneration Research

Abstract

Salamanders are important vertebrate model organisms in regenerative medicine. In this application, we proposed to enhance research resources for the Mexican axolotl (Ambystoma mexicanum) by innovating an approach to sequence their large 32,000,000,000 base pair genome. We successfully developed methods to isolate and sequence whole axolotl chromosomes, and published a manuscript in a leading journal that describes our approach and initial characterization of the axolotl genome. Thus, we accomplished the objectives of our project, laying the ground work for developing a full axolotl genome assembly.

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Document Details

Document Type
Technical Report
Publication Date
May 06, 2016
Accession Number
AD1057905

Entities

People

  • S. Randal Voss

Organizations

  • University of Kentucky

Tags

Communities of Interest

  • Human Systems

DTIC Thesaurus Topics

  • Abstracts
  • Agreements
  • Assembly
  • Chromosomes
  • Department Of Defense
  • Dna Sequence Analysis
  • Engineering
  • Genetic Markers
  • Genetics
  • Genome
  • Genomics
  • Mathematics
  • Regenerative Medicine
  • Sequences
  • Statistics
  • Stem Cells
  • Students

Fields of Study

  • Biology

Readers

  • Aquatic Ecology
  • Molecular Biology and Genetics
  • Technical Research and Report Writing.