A New Paradigm for Ovarian Sex Cord-Stromal Tumor Development

Abstract

Transforming growth factor beta (TGFB) signaling regulates fundamental reproductive events via TGFB receptor complexes (TGFBR1/TGFBR2) and downstream SMAD proteins. To determine potential effect of overactivation of TGFB signaling in distinct cellular compartments, we generated a mouse model containing a constitutively active TGFBR1 using growth differentiation factor 9 (Gdf9)-Cre (termed TGFBR1-gCA). We showed that sustained activation of TGFBR1 disrupts folliculogenesis via affecting ovarian reserve and follicle growth/development. Ovarian tumor tissues from TGFBR1-gCA mice were positive for granulosa cell markers. RNA-Seq analysis using ovarian RNA from TGFBR1-gCA mice and controls identified a number of genes associated with folliculogenesis, oogenesis, proliferation, and differentiation. Histological and molecular analyses provided evidence of overactivation of TGFB signaling in ovarian granulosa cell compartment. The mouse model may be further exploited to define the cellular and molecular mechanisms of TGFB/activin downstream signaling in granulosa cell tumor development.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 2018
Accession Number
AD1058206

Entities

People

  • Qinglei Li

Organizations

  • Texas A&M University

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Apoptosis
  • Biomedical Research
  • Cell Physiological Processes
  • Chemical Synthesis
  • Chemistry
  • Data Analysis
  • Data Mining
  • Germ Cells
  • Growth Factors
  • Medical Personnel
  • Neoplasms
  • Pcr Testing
  • Peptide Growth Factors
  • Peptides
  • Proteins
  • Students

Fields of Study

  • Biology

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