Improving Protein Stability and Activity Using Zwitterionic Peptides

Abstract

This work established the use of polypeptides composed of repeating glutamic acid (E) and lysine (K) (poly(EK)) for the purpose of increasing protein stability. The addition of poly(EK) to the primary sequence at the C-terminus of a model beta-lactamase protein and a destabilized TEM-19 demonstrated significant ability in increasing protein stability while maintaining bioactivity. The use of fusion protein products allows for a one-step protocol resulting in a homogeneous product, a significant advantage over polymer-based approaches.

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Document Details

Document Type
Technical Report
Publication Date
Mar 13, 2017
Accession Number
AD1058598

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  • Shaoyi Jiang

Organizations

  • University of Washington

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DTIC Thesaurus Topics

  • Abstracts
  • Agreements
  • Chemical Stability
  • Chemical Synthesis
  • Chemistry
  • Department Of Defense
  • Engineering
  • Ethylene Glycol
  • Genetic Engineering
  • Glutamic Acid
  • Information Operations
  • Materials
  • Mathematics
  • Molecular Dynamics
  • Molecular Dynamics Simulations
  • Phase
  • Polymers
  • Proteins
  • Sequences
  • Simulations
  • Solid Phases
  • Structural Properties
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  • Molecular and Cellular Biochemistry