The Urinary Fungal Mycobiome and Host Responses in Patients with Interstitial Cystitis
Abstract
While subject recruitment and sample acquisition remain challenging, we have 150 participants enrolled to date, have identified distinctive bacterial community types with a preliminary relationship to dysbiosis, and developed new techniques for identifying fungal species. We have successfully extracted both fungal and bacterial DNA from the catheterized urine samples and confirmed this through both quantitative polymerase chain reaction (qPCR) and gel electrophoresis. We are refining classification of bacterial communities found in both controls and individuals diagnosed with Interstitial Cystitis and are confirming the connection between specific types and pelvic pain/disease. Analysis of fungal communities has proved more challenging, requiring us to develop specific probes that will allow us to complete this analysis. We have verified the accuracy of these techniques and will proceed to apply them to our samples.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2018
- Accession Number
- AD1059189
Entities
People
- A. L. Ackerman
Organizations
- Cedars-Sinai Medical Center