Loss of ZDHHC-Mediated Scribble Palmitoylation Disrupts Cell Polarity and Promotes Prostate Cancer Progression

Abstract

Progression and metastasis of prostate cancers (PCs) are major therapeutic challenges, with the underlying mechanisms remaining unclear. The apical-basal polarity of epithelial cells plays critical roles in regulating normal cell migration and proliferation in prostate. Loss of cell polarity leads to tissue disorganization, uncontrolled proliferation and migration, hallmarks of prostate cancer progression and metastasis. ZDHHC7-mediated palmitoylation of Scribble is critical for cell polarity and metastasis. In the first year of the study, we have analyzed the palmitoylation levels of Scribble in various prostate cancer cell lines, at different progression stage. We analyzed the localization of Scribble in these cells, and correlated its palmitoylation levels. We have generated ZDHHC7 gain-of-function cell lines, and evaluated the effects on Scribble.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2018
Accession Number
AD1059203

Entities

People

  • Xu Wu

Organizations

  • Massachusetts General Hospital

Tags

DTIC Thesaurus Topics

  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Fatty Acids
  • Health Services
  • Intercellular Junctions
  • Medical Personnel
  • Peptides
  • Proteins
  • Proteomics

Fields of Study

  • Chemistry

Readers

  • Geochemistry
  • Oncology (Cancer Research).