Aeromonas caviae Secreted Proteases as a Potential Mechanism of Avian Influenza A Virus Persistence
Abstract
Proteolytic cleavage of hemagglutinin is required for cell entry and plays a role in influenza virus pathogenicity. Little is known about the role of the bacterial flora of birds on the persistence and replication of avian influenza virus. Previous studies identified 56 caseinolytic-secreting bacterial species capable of cleaving hemagglutinin in a trypsin-like manner. The goal of this study was to identify and characterize Aeromonas hydrophila secreted proteases. We separated secreted proteases ion via ion-exchange and size-exclusion chromatography cell-free supernatants and fractions exhibiting proteolytic activity were sequenced for protein identification. Seven caseinolytic proteins were selected for isolation and characterization to determine the role of these respective contributors in virus activation/inactivation. These proteolytic activities may reflect ecological and host environments in which avian influenza viruses persist and replicate. Complete understanding of proteases found in natural sites of infection is necessary to derive insight into mechanisms of pathogenicity, mechanisms of perpetuation, and ease of cross-species transmissibility.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 16, 2018
- Accession Number
- AD1059603
Entities
People
- Brook Kunzelman
- Casey Guardia
- John A White
- Marcus King
- Micheal Mechikoff
Organizations
- United States Air Force Academy