Optimization of Wound Healing to Limit Infection
Abstract
The overall goal of this project remains to develop an effective clinical strategy employing immune-regulation as a means to reverse altered immune function in stressed combatants and thus reduce infection and restore an optimal wound healing response. Two models are being explored. In the studies being pursued here in the Laboratory of Tissue Repair at the VCU Medical Center immune suppression was achieved using a long acting form of methyl prednisolone called DepoMedrol. Open wounds were then inflicted in either rats (Male Sprague-Dawley) or mice (male CD-1) and then Androstenediol was administered to determine if the impaired healing response would return to normal. Blood was collected for total white cell analyses and their state of activation (CD 11b and CD 45 surface markers). Interleukins such as IL-2, 6, 8 and 10, key inflammatory signals, were also followed. Although some modulations in the white cell counts and state of activation were observed, no significant wound healing response was observed in the absence of an infection.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2010
- Accession Number
- AD1060276
Entities
People
- David G. Baer
- Kevin R. Ward
- Robert F. Diegelmann
Organizations
- Virginia Commonwealth University