Role of SRC 3delta4 in the Progression and Metastasis of Castration Resistant Prostate Cancer. Addendum
Abstract
SRC-34, an N-terminus deletion isoform of steroid receptor coactivator (SRC-3), was shown to act as a signaling adaptor of EGF signaling. Its role in prostate cancer (PCa) progression is unclear. As such, we tested whether SRC-34 coactivates AR in an androgen-independent manner and promotes prostate cancer cell growth and invasiveness in response to EGF signaling. We have found that EGF stimulated the interaction of SRC-34 with AR, the recruitment of SRC-34 to the promoters of AR target genes, and AR target genes transcription in androgen-depleted culture conditions. In addition, SRC-34 promotes androgen-independent prostate cancer cell growth and invasion, in which EGF-induced phosphorylation of SRC-34 plays a critical role. However, SRC-34 did not affect AR-mediated prostate tumor growth and progression in xenograft mouse models. Taken together, these results demonstrate that SRC-34 acts as a coactivator of AR and regulates the transcription of AR target genes and prostate cancer cell growth and invasion in response to EGF signaling in cultured cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2018
- Accession Number
- AD1060286
Entities
People
- Weiwen Long
Organizations
- Wright State University