Unlocking Barriers to DNA Vaccine Immunogenicity: A Cross Species Analysis of Cytosolic DNA Sensing in Skeletal Muscle Myocytes

Abstract

DNA vaccine technology holds great promise as a platform for developing vaccines against both emerging and established global pathogens. Despite this potential, significant challenges impede the capacity of DNA vaccines to prevent disease in humans. Foremost amongst these, is the gap between remarkable results obtained in pre-clinical mouse models and relatively modest immunogenicity observed in humans. The present work is testing the hypothesis that skeletal muscle myocytes sense cytosolic DNA and elaborate an inflammatory response to DNA vaccines and that species-specific differences in the cytosolic DNA sensing system bring about divergent inflammatory responses inhuman versus mouse skeletal muscle myocytes.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2018
Accession Number
AD1060308

Entities

People

  • Gerasimos Zaharatos

Organizations

  • Jewish General Hospital

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Antibodies
  • Antigens
  • Biological Factors
  • Biomedical Research
  • Cell Line
  • Cells
  • Chemistry
  • Confocal Microscopy
  • Data Analysis
  • Detection
  • Detectors
  • Dna Biosensors
  • Emerging Technology
  • Experimental Design
  • Gene Expression
  • Health Services
  • Immunogenicity
  • Infectious Diseases
  • Medical Personnel
  • Muscles
  • Platforms
  • Proteins
  • Skeletal Muscle
  • Training
  • Transcription Factors
  • Vaccines

Fields of Study

  • Biology

Readers

  • Immunology and Pathology
  • Molecular Genetics
  • Systems Analysis and Design

Technology Areas

  • Biotechnology