Characterization of Virucidal Resistance of DNA Viruses
Abstract
The objective of this study was to compare the virucidal effectiveness of various disinfectants on the Vaccinia virus (VACV) and the Helicoverpa zea single-capsid nucleopolyhedrosis virus (HzSNPV) to determine whether the HzSNPV could be used as a simulant for the orthopox Variola virus, the causative agent of smallpox. VACV and HzSNPV were exposed to five commonly used disinfectants and three detergents. Results showed that VACV and HzSNPV exhibited similar responses to the virucidal treatments, although differences were observed as functions of concentration and exposure time. VACV showed greater susceptibility to treatment at low concentrations and lower exposure times. Host insect cells also showed direct toxic responses to some of the aldehyde and detergent solutions, and these responses were not observed in the host Vero cells that were used to propagate the VACV. We concluded that the HzSNPV demonstrated sufficient correlation with the response of the VACV to disinfectant treatment, which further warrants consideration of HzSNPV as an effective simulant for the Variola strain of viruses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2018
- Accession Number
- AD1060343
Entities
People
- Amanda Chambers
- Charles Hong
- Darrel Menking
- Janna Madren-whalley
- Patricia E. Buckley
- Roy Thompson
Organizations
- Edgewood Chemical Biological Center