Nanoink Printed Amperometric Immunosensor for Rapid and Inexpensive Screening of Tuberculosis

Abstract

The project aims to develop a point-of-care diagnostic platform for tuberculosis diagnosis. The sensor detects Mycobacterium tuberculosis (MTB; H37Ra strain) cells and MPT-64 antigen spiked in human sputum samples. Single walled carbon nanotubes are used as a sensing element for detection of target analytes. In the project period, various SWCNTs-based immunosensors were designed and fabricated. The fabrication and detection protocol was optimized. For specific detection, antibodies to MPT-64 were generated and characterized. Through affinity purification, the antibodies became more sensitive and specific to target analytes. Using the sensor, the detection limit was 10(exp 6) CFU/mL for BCG and 100 ng/mL for MPT-64. In the next project period, the detection protocol will be further optimized to achieve detection limit of 10(exp 3) CFU/mL (or equivalent concentration to MPT-64).

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 2018
Accession Number
AD1060406

Entities

People

  • Clement Furlong
  • Jae-Hyun Chung
  • Jong-hoon Kim

Organizations

  • University of Washington

Tags

Communities of Interest

  • Biomedical
  • Sensors

DTIC Thesaurus Topics

  • Biosensors
  • Carbon Nanotubes
  • Dacron
  • Detection
  • Detectors
  • Fabrication
  • Fullerenes
  • Manufacturing
  • Materials
  • Materials Processing
  • Materials Science
  • Molecules
  • Nanomaterials
  • Nanotechnology
  • Point-Of-Care Diagnostic Testing
  • Students
  • Surface Tension

Readers

  • Immunology
  • Nanocomposite Materials Science
  • Oncology and Biomarker-Based Cancer Detection.