Characterization of Clustered CTCs to Eliminate Breast Cancer Metastasis
Abstract
This program aims to examine the role of platelets and IL-11 in breast cancer tumor cell clustering and metastasis to the organs such as lung. In year 1, we determined that 5 days ofIL-11 treatment to C57BL/6, but not SCID or balb/c, mice resulted in a 2-3-fold increase in platelet counts. Thus, only IL-11induced thrombocytosis only occurs in C57BL/6 mice. In year2 of the program, we then had to create a murine model for metastatic breast cancer inC57BL/6 mice. After establishing the institutional protocols to 1) label C57BL/6 tumor(EO771-LMB cells) with luciferase, 2) obtain Case Western Reserve University IACUC approval for these studies, 3) obtain Case Western Reserve University Institutional Biosafety Committee (IBC) approvals to inject stably transfected tumor cells into mice, and 4) Obtainre-approvals from DOD (ACURO) of our revised animal protocols, we began experiments using a syngeneic murine mammary cancer tumor in the C57BL/6.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2018
- Accession Number
- AD1061767
Entities
People
- Alvin H. Schmaier
Organizations
- Case Western Reserve University