Assessing Aquatic Toxicity of Insensitive Munitions (IM) compounds Using Larval Fathead Minnows (Pimephales promelas)
Abstract
This Scientific Operating Procedure (SOP) provides a standard method for assessing the aquatic toxicity of insensitive munitions (IM) formulations or their single constituents using the larval stage of the freshwater fish Pimephales promelas (fathead minnow). The sole endpoint for the acute, 48-hour non-renewal test using P. promelas is survival. Exposure is conducted using larval P. promelas four to ten days old. The larval fish are exposed to a decreasing concentration series of IM compounds (e.g., 100%, 50%, 25%, 12.5%, and 6% of the highest concentration) and to a negative control at 25 1C in 300 mL glass beakers containing 200 mL of test solution. For each concentration, four replicate beakers, each containing ten larvae are used. Water quality parameters are measured at the beginning and termination of the test. The tests are only valid when the mean survival for the control treatment is 90% or greater. Instructions and requirements are provided for handling P. promelas before and during the toxicity test, preparing aqueous mixtures, initiating and terminating 48-hour tests, maintaining appropriate test conditions, making necessary observations, water quality measurements, assessing the survival endpoint and obtaining samples for chemical analysis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2018
- Accession Number
- AD1063077
Entities
People
- Guilherme R Lotufo
- Jacob K. Stanley
- Mark L. Ballentine