Interaction of Bacterial Metabolites with Fluorescent Synthetic DNA
Abstract
This project was aimed at studying and developing polyfluorophores on a DNA backbone for sensitivity to the environment; ultimately, to sense organic bacterial metabolites in the air. Overall, we made substantial progress in synthesizing new classes of fluorescent DNA monomers and DNA polyfluors and in finding new ways to put them on solid supports. We synthesized and studied a new class of DNA fluorophores based on aggregation-induced emission, which we hypothesized might be particularly environmentally sensitive. Moreover, during our studies we identified a new type of dye that undergoes unusual photo bleaching with color changes, which may lead to new tools for biology and microscopy. Finally, very recently we completed a 1296-member library of DNA-polyfluorophores, and we hope that it will be able to distinguish organic compounds in the air. Although the project ran out of time before we could begin to test this library against bacterial colonies, the work has allowed us to discover novel DNA-dye structures and novel mechanisms of sensing and switching. We hope to test our recently completed ODF library with bacterial metabolites in the future.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 11, 2016
- Accession Number
- AD1063352
Entities
People
- Eric T. Kool
- Niaz Banaei
Organizations
- Stanford University