Detection and Characterization of Treatment Emergent Neuroendocrine Prostate Cancer Using Hyperpolarized 13C Magnetic Resonance
Abstract
The goal of this proposal is to discriminate biologically divergent treatment-emergent neuroendocrine prostate cancer (NEPC) from advanced adenocarcinoma based on the metabolic profile of NEPC tumors and to use real-time changes in metabolism to monitor therapeutic efficacy by using novel hyperpolarized (HP)(sup 13)C metabolic imaging techniques. In the 1st year of funding, our major activities focused on collating preliminary studies for Aims 1 and 2 to establish feasibility. To this end for Aim 1, the probes (glutamine and acetate) were optimized for hyperpolarization technique and tested in cells and baseline TRMAP studies. Correlative measures of NEPC presence were developed using ELISA and serum enzyme activities in addition to IHC quantification. Furthermore preliminary metabolic profiling of TRMAP tumors showed altered substrate utilization with the onset of CRPC further strengthening our hypothesis of metabolic adaptations with treatment resistance.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2018
- Accession Number
- AD1063802
Entities
People
- Renuka Sriram
Organizations
- University of California, San Francisco