Neuropathology and Immune Biomarker Discovery in a Rat Model of Alzheimer's Disease,TgF344-AD, with Single or Repetitive Traumatic Brain Injury
Abstract
Purpose: The major goals of this project are to develop better models to investigate mechanisms by which traumatic brain injury (TBI) is a risk factor for developing Alzheimer's disease (AD) and related neurodegenerative diseases, including chronic traumatic encephalopathy (CTE). To accomplish these goals, we are using wild-type control rats and transgenic (Tg) AD rat models to conduct and contrast two paradigms of closed-head TBI, and one paradigm of repetitive moderate blast TBI, after which cohorts of varying ages post injury are studied for immune system changes within both the periphery and central nervous system (CNS) to determine their role in AD pathogenesis. Scope: The study is limited to developing and characterizing these new TBI-AD models, and does not incorporate treatments or translational efforts for human clinical settings. Major Findings: After receiving ACURA approval on 1-4-18 to begin animal work, we have been actively expanding the Tg AD rat colony, using all male progeny for breeding, to thereafter age and use within 12-month-old TBI cohorts. Meanwhile, we ordered 60 control rats that are now aged and scheduled for experimental procedures next month. The controlled cortical impactor was placed in the vivarium and validated, accessory equipment and supplies were obtained, and, to date, four 12-month-old rats have completed experimental procedures with biospecimens collected.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2018
- Accession Number
- AD1063953
Entities
People
- Huntington Potter
Organizations
- Regents of the University of Colorado