Assessment of Post Remediation Performance of a Biobarrier Oxygen Injection System at a MTBE Contaminated Site
Abstract
This Environmental Security Technology Certification Program (ESTCP)-funded project was performed to evaluate the long-term performance of monitored natural attenuation (MNA) at a site where natural attenuation of methyl tert-butyl ether (MTBE) is being used as a polishing step following in situ bioremediation. To achieve this goal, two primary objectives were identified: -Evaluate the current microbial activity supporting natural attenuation of MTBE using a combination of conventional contaminant concentration data and geochemistry trend analyses and advanced molecular biological tools (MBTs), including metaproteomics and metagenomics. -Assess the long-term impact of the biobarrier system on formation permeability. In addition to evaluating data collected using conventional monitoring techniques, this project applied metagenomics and metaproteomics to improve the understanding of long-term impacts of the remedy on biodegradation at the site. Use of these advanced MBTs for quantification and detection of biomarkers, especially deoxyribonucleic acid (DNA) and peptides (protein fragments) in environmental samples has been rapidly expanding over the last few decades.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 2018
- Accession Number
- AD1064260
Entities
People
- Craig Bartling
- Heather V. Rectanus
- Kate H. Kucharzyk
- Kenda Neil
- Pamela Chang
- Tanwir Chaudhry
Organizations
- Naval Facilities Engineering Systems Command