Systems Biology of Immune Response to Live and Inactivated Dengue Virus Vaccines

Abstract

The objective of this project is to elucidate the immunological mechanisms induced by live attenuated and purified inactivated dengue virus vaccines administered in a heterologousprime-boost regimen. Innate and adaptive (T and B cell) responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach.During the first project year, IRB approvals, inter-institutional agreements, and measurements of dengue virus-specific neutralizing antibody titers and frequencies of cytokine-producing CD4 and CD8 T cells were completed. Flow cytometry of cellular activation and measurement of serum cytokine levels were completed on a subset of subjects. Pilot studies were done for RNA extraction and analysis of B cells and T cell repertoires.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2018
Accession Number
AD1064691

Entities

People

  • Jeffrey R. Currier

Organizations

  • Geneva Foundation

Tags

DTIC Thesaurus Topics

  • Antibodies
  • B Lymphocytes
  • Biology
  • Biomedical Research
  • Blood
  • Cells
  • Computational Biology
  • Cytokines
  • Data Analysis
  • Databases
  • Department Of Defense
  • Engineering
  • Gene Expression
  • Governments
  • Immunity
  • Immunochemistry
  • Institutional Review Board
  • Lymphocytes
  • Medical Personnel
  • Pilot Studies
  • Rna Sequence Analysis
  • Systems Biology
  • Vaccination
  • Vaccines
  • Virus Diseases

Fields of Study

  • Biology
  • Medicine

Readers

  • Clinical Trial Research.
  • Immunology
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech