Systems Biology of the Immune Response to Live and Inactivated Dengue Virus Vaccines

Abstract

The objective of this project is to elucidate the immunological mechanisms induced by live-attenuated and purified inactivated dengue virus vaccines administered in a heterologous prime-boost regimen. Innate and adaptive (T and B cell) responses will be measured using molecular and cellular approaches and the data analyzed using a systems biology approach. During the second project year, subjects were re-consented for genetic analyses. RNA isolation was completed and library preparation for RNA-seq was performed. Pipelines for generation and analysis of single-cell gene expression and T cell receptor data were established. HLA typing was initiated. Project databases were configured and additional data tables were uploaded.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 2018
Accession Number
AD1064692

Entities

People

  • Alan L. Rothman
  • Jeffrey R. Currier

Organizations

  • University of Rhode Island

Tags

DTIC Thesaurus Topics

  • B Lymphocytes
  • Biology
  • Biomedical Research
  • Blood
  • Cells
  • Computational Biology
  • Data Analysis
  • Databases
  • Department Of Defense
  • Engineering
  • Gene Expression
  • Immunity
  • Immunochemistry
  • Institutional Review Board
  • Lymphocytes
  • Medical Personnel
  • Pipelines
  • Rhode Island
  • Rna Sequence Analysis
  • Systems Biology
  • Universities
  • Vaccination
  • Vaccines
  • Virus Diseases

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and genetic basis of cancer.
  • Virology (or Medical Virology).

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech