Assessment of Chemical Exposure in Multi-animal and Cell Models Using Multiple Omics Platform

Abstract

This work supports the U.S. Army Medical Research and Materiel Commands (USAMRMC) Biotechnology High Performance Computing Software Applications Institute (BHSAI) in its efforts to construct predictive models to assess liver, kidney and heart damages of chemical toxicants and drugs. To validate the platform, metabolic changes induced by toxicant exposure is supposed to be experimentally identified from the comprehensive analysis of in vivo transcriptomic data collected from the liver, kidney, heart, plasma, and urine of animals. To translate the results obtained in animal models to human, multiple species of animals, rats and guinea pigs, were used. To dissect the primary from the secondary transcriptomic response of liver, kidney and heart cells, resulted from altered metabolism of other organs/tissues, to toxicant exposure, transcriptomic analysis was performed on primary hepatocytes, renal tubular epithelial cells, cardiacmyocytes originated from rats. We examined toxic effect of mercury chloride and thioacetamide.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 2018
Accession Number
AD1064813

Entities

People

  • Masakazu Shiota

Organizations

  • Vanderbilt University

Tags

DTIC Thesaurus Topics

  • Animals
  • Application Software
  • Biomedical Research
  • Blood
  • Body Weight
  • Cardiac Arrhythmias
  • Cardiovascular System
  • Cells
  • Chemistry
  • Chlorides
  • Epithelial Cells
  • Health Care
  • Health Services
  • High Performance Computing
  • Poisoning
  • Rodents
  • Veins

Fields of Study

  • Biology

Readers

  • Military Science and Technology Research and Modernization.
  • Molecular and Cellular Biology
  • Toxicology/Environmental Toxicology

Technology Areas

  • Biotechnology