Hydroxocobalamin with Cardiopulmonary Resuscitation Compared to Cardiopulmonary Resuscitation Alone in Protection of Neuronal Cell Death Produced by Global Brain Ischemia Induced by Cardiac Arrest in Swine (sus scrofa)
Abstract
Purpose: To evaluate the effects of hydroxocobalamin (HOC) in post cardiac arrest swine 24-72hrs post injury global and microscopic neurohistology. Methods: Swine (22-32kg) were anesthetized, intubated and instrumented for continuous monitoring. Ventricular fibrillation (VF) was induced followed by BLS and ACLS until return of spontaneous circulation (ROSC). Monitored for 60 mins post ROSC and then recovered and observed for 72hrs. After observation period, animals were euthanized and brains immediately removed and placed in 10 NBF for fixation. Results: The study was not completed, closed due to triennial renewal date. Based on the work that was done, an 8 minute cardiac arrest model was survivable and produced "rare neuornal necrosis in the CA1 area of the hippocampus" per staff pathologist. Conclusions: After much discussion between the study team and collaborators, moving forward in the next phase, the primary focus will be the neurological function of the treated animal versus the control animals in a 8 minute cardiac arrest model.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 06, 2018
- Accession Number
- AD1064827
Entities
People
- Joseph K Maddry
- Kavsie Canellis
- Kaysie Canellis
- Kole Spaulding
- Maria G. Castaneda
- Normalynn Bebarta
- Normalynn Garrett
- Susan M. Boudreau
- Vikhyat S Bebarta
Organizations
- 59th Medical Wing