Clinical Significance and Mechanistic Insights into Ovarian Cancer Mitochondrial Dysfunction
Abstract
Our work addresses the hypothesis that mitochondrial dysfunction plays a role in the etiology and chemoresistance of epithelial ovarian cancers. We are focusing on the role of the fission protein Drp1 in this context. Specifically we discovered that expression of a low molecular weight Drp1 variant is associated with mitochondrial fission/fusion defects. Mass spec and RNA sequencing analysis has revealed that the low molecular weight (LMW) isoform of Drp1 does not arise as a consequence of alternate transcriptional promoter use, but may be dependent on an alternate variable domain and C-terminal truncation. We are interrogating the role of short Drp1 as a dominant negative fission protein and are investigating its binding affinity to mitochondria and interaction with fission accessory proteins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 31, 2018
- Accession Number
- AD1064854
Entities
People
- Dong Hoon Shin
- Nadine Hempel
Organizations
- Pennsylvania State University