Photonic reagents for concentration measurement of fluorescent proteins with overlapping spectra
Abstract
By exploiting photonic reagents (i.e., coherent control by shaped laser pulses), we employ Optimal Dynamic Discrimination (ODD) as a novel means for quantitatively characterizing mixtures of fluorescent proteins with a large spectral overlap. To illustrate ODD, we simultaneously measured concentrations of in vitro mixtures of Enhanced Blue Fluorescent Protein (EBFP) and Enhanced Cyan Fluorescent Protein (ECFP). Building on this foundational study, the ultimate goal is to exploit the capabilities of ODD for parallel monitoring of genetic and protein circuits by suppressing the spectral cross-talk among multiple fluorescent reporters.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 16, 2016
- Accession Number
- AD1068812
Entities
People
- Alexei Goun
- Ali O. Er
- Denys I Bondar
- Herschel A. Rabitz
- Zachary Quine
Organizations
- Princeton University