Matrix-Free Assisted Laser Desorption Ionization Using Metal-Organic Frameworks
Abstract
We studied the ionization mechanism and determined the factors that affect the charge-transfer process during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). We addressed the binding affinity issue between metal-organic frameworks (MOFs) and analytes using low-frequency Raman spectroscopy (LFRS) to determine inter- and intramolecular changes between crystalline and amorphous states of MOFs with different analytes. These analytes were classified as acidic or basic compounds. As soon as we understand the basic properties of MOF/analyte mixtures, we will investigate how to design MOFs that can enhance ionization efficiency for a wide range of compounds during MALDI-MS analysis. This knowledge will be used to design and develop a reliable, functionalized MOF substrate that can desorb biomolecules and selectively capture analytes of interest. We addressed the potential inter- and intramolecular changes for MOF/analyte mixtures using LFRS and MALDI-MS techniques. The MOFs used in the LFRS were UiO-66-COOH and UiO-66-NH2, and the analytes were pyridine, benzoic acid, cytidine, lauric acid, and guanine. More MOFs than those tested by the LFRS technique were used for the MALDI-MS analyses. Most of the MOFs were obtained from internal sources, either through synthesis or by leveraging from already-funded projects that use MOFs.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2019
- Accession Number
- AD1069268
Entities
People
- Gregory W. Peterson
- Jared B DeCoste
- Rabih E. Jabbour
- Yousef Jabaji