Novel Surface Phenomena
Abstract
Major Goals: The major goals in this research project are several-fold: (1) developing a mechano-chemical theory to treat different types of single molecule experiments on biomolecular motors, initially those on F1-ATPase involved in pumping protons and other ions across membranes, (2) extending the theory of sum frequency generation experiments for studying the effect of hydrogen bonding at water-organic interfaces on the rates of organic reactions, such as cycloaddition reactions, (3) understanding wasteful electron-hole recombination processes involved in photo-excited perovskite materials of solar cell interest, in particular Auger recombination, and (4) understanding fluorescence blinking of photoexcited CdSe and other semiconductor nanoparticles and the dependence of the rate of energy-wasteful nonradiative recombination of electrons and holes on the size of the nanoparticle. In topic (1) there are now, in addition to many bulk system experiments, different types of single molecule experiments, such as stalling experiments, in which the rotary biological motor is stalled at various angularpositions by magnetic tweezers and then after different times allowed to rotate forward or backward to stable positions and relevant rate constants are obtained from those data. There are also controlled rotation experiments, in which the motor is rotated at a constant angular velocity that is controlled by magnetic tweezers. To monitor the latter experiments the ATP (adenosine triphosphate) in F1-ATPase studies is replaced by a modified fluorescent ATP. The latter serves to determine whether a site in the ATPase is occupied (the attached dye in the ATP in the ATPase then fluoresces) or not occupied (the ATP and its attached dye are then in solution and there is no fluorescence). From the latter information one obtains rates of various processes occurring in this motor.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 30, 2018
- Accession Number
- AD1070319
Entities
People
- Rudolph A. Marcus
Organizations
- California Institute of Technology