Autologous Hematopoietic Stem Cell Transplantation to Prevent Antibody-Mediated Rejection After Vascularized Composite Allotransplantation
Abstract
The investigators successfully performed initial control experiments in Group 1, unsensitized animals applying the proposed induction andmaintenance immunosuppressive regimen to assess its safety and feasibility for maintaining VCA allograft survival in this model. Successful VCA (i.e. swine heterotopic hind limb) transplantation in Group 2 were performed across a full SLA mismatch in the setting of donor skin sensitization. The animals were treated with non-myeloablative total body irradiation and thymic irradiation two days prior to transplantation and with continues calcineurin inhibitor-based immunosuppressive maintenance therapy using tacrolimus at target trough levels of 10-20 ng/ml for 30 days.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2018
- Accession Number
- AD1070365
Entities
People
- Gerald Brandacher
Organizations
- Johns Hopkins University